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| Fernandes,Eloise Helena; Prioli,Alberto José; Scapim,Carlos Alberto; Schuster,Ivan; Vieira,Elisa Serra Negra; Amaral Júnior,Antônio Teixeira do; Moterle,Lia Mara. |
| A primeira etapa de um programa de transformação genética de plantas é o estabelecimento de um protocolo de regeneração de plantas, a partir de cultura de tecidos. A regeneração de plantas pode ser conseguida por meio de organogênese ou embriogênese. A embriogênese somática é dependente do genótipo das plantas utilizadas e a identificação de genótipos mais responsivos a este método de regeneração resulta em maior eficiência da técnica. O trabalho teve como objetivo identificar genótipos de milho com maior capacidade de produção de embriões somáticos e regeneração de plantas. Foram investigados 11 genótipos (linhagens e híbridos). As culturas foram obtidas a partir de embriões imaturos, inoculados em meio N6, suplementado com 690mg L-1 de prolina e 10mM de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Cultura de tecidos; Regeneração. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000900031 |
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| Rimoldi,Fabrício; Vidigal Filho,Pedro Soares; Kvitschal,Marcus Vinícius; Gonçalves-Vidigal,Maria Celeste; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Costa,Tiago Ribeiro da. |
| In this work the genetic divergence among 14 sweet cassava cultivars was estimated by their morphological agronomic traits and RAPD molecular markers. The Tocher cluster analysis and the Nearest Neighbor Method were applied. The most dissimilar cultivars were Pão and Guaíra, Fécula Branca and Pão, and Pão and Caipira, while the most similar cultivar were the Fécula Branca and Branca 1, Branca 3 and Branca 1, and Guaíra and Branca 1. The Jaccard's coefficient showed that the most similar cultivars were Guaíra and Quarenta Quilos, while the most dissimilar were Branca 3 and Amarela da Rama Cinza. The divergence analysis indicated that promising crosses could be made between the Branca 3 cultivar and the Pão, Amarela 1, Fécula Branca and Amarela 2 cultivars... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Manihot esculenta; Multivariate analysis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600025 |
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| Bignotto,Thaís Souto; Maniglia,Thiago Cintra; Gomes,Vivian Nunes; Oliveira,Isadora Janolio de; Agostinho,Carlos Sérgio; Prioli,Sônia Maria Alves Pinto; Prioli,Alberto José. |
| Abstract Mitochondrial molecular markers (DNA sequences of D-loop, cytochrome b and cytochrome c oxidase I) were employed to characterize populations of the piranha Serrasalmus maculatus from Upper Paraná, Upper Paraguay and Tocantins River basins. D-loop sequences of S. maculatus population from Paraná-Paraguay River basin exhibited tandem repeats of short motifs (12 base pairs) and variable numbers depending on specimens, accounting for length variation. Concatenated mitochondrial sequences suggested that S. maculatus encompasses different mitochondrial DNA lineages. Although sampling was restricted to three river basins, phylogenetic analysis clearly indicated that the species currently recognized as S. maculatus presents high genetic variability.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptic species; Species delimitation; D-loop; Cytochrome b; Cytochrome c oxidase I. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100801 |
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| Panarari-Antunes,Renata de Souza; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Galdino,Alexsandro Sobreira; Julio Junior,Horácio Ferreira; Prioli,Laudenir Maria. |
| This study aimed to evaluate the genetic variability of B. orbignyanus in cultivated and natural populations of the Upper Paraná River, using molecular RAPD markers and mtDNA control region. Specimens were collected in the Paraná River and in the Piracema fish farm in Maringá, State of Paraná, Brazil. RAPD primers produced 82 loci with consistent expression. The population from the Paraná River showed 28 polymorphic loci, whereas the population from the fish farm presented only 12. Data revealed genetic differentiation between the two populations, although not very pronounced. These results were corroborated by the principal coordinate analysis and by neighbor-joining clustering. The alignment of the D-loop sequences of B. orbignyanus indicated the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fish; Endangered; RAPD; MtDNA; Polymorphism. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400025 |
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